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Publication date: February 2017Source:Journal of Archaeological Science, Volume 78 Author(s): A.C. Colonese, A. Lucquin, E.P. Guedes, R. Thomas, J. Best, B.T. Fothergill, N. Sykes, A. Foster, H. Miller, K. Poole, M. Maltby, M. Von Tersch, O.E. CraigPoultry products are rarely considered when reconstructing pottery use through organic residue analysis, impinging upon our understanding of the changing role of these animals in the past. Here we evaluate an isotopic approach for distinguishing chicken fats from other animal products. We compare the carbon isotopes of fatty acids extracted from modern tissues and archaeological bones and demonstrate that archaeological bones from contexts associated with pottery provide suitable reference ranges for distinguishing omnivorous animal products (e.g. pigs vs. chickens) in pots. When applied to pottery from the Anglo-Saxon site of Flixborough, England, we succeeded in identifying residues derived from chicken fats that otherwise could not be distinguished from other monogastric and ruminant animals using modern reference values only. This provides the first direct evidence for the processing of poultry or their products in pottery. The results highlight the utility of ‘in-situ’ archaeological bone lipids to identify omnivorous animal-derived lipids in archaeological ceramic vessels.

Publication date: Available online 11 January 2017Source:Journal of Archaeological Science Author(s): Dolores R. Piperno, Anthony J. Ranere, Ruth Dickau, Francisco AceitunoThe various theoretical approaches advanced over the past 50 years to explain the origins of agriculture have prompted much discussion and debate. Most recently, controversy has arisen concerning the utility of two Darwinian approaches; namely, cultural niche construction (CNC) and human behavioral ecology-derived optimal foraging theory (OFT). Recent papers advocate for the primacy of cultural niche construction, calling for optimal foraging approaches to be all but disregarded in the quest to explain how and why foragers became farmers (Smith, 2015, 2016; Zeder, 2015, 2016). In particular, it is claimed that archaeological, paleo-environmental, and paleontological evidence from the Neotropics of northern South America fail to meet predictions derived from OFT theory, while predictions said to be derived from CNC-based approaches are supported (Smith, 2015, 2016; Zeder, 2015). However, a number of misreadings of the northern South America evidence are made in those discussions, while some pertinent literature is not considered. In this paper we discuss these misreadings and provide a clear re-articulation of the original data and interpretations, finding support for OFT predictions. Our re-evaluations of OFT and CNC further suggest they can, in fact, be complimentary explanatory approaches.

Publication date: February 2017Source:Journal of Archaeological Science, Volume 78 Author(s): Gilliane Monnier, Ellery Frahm, Bing Luo, Kele MissalThe analysis of residues on stone tools can yield important insights into the tool-using behaviors of Paleolithic hominins. The ambiguity of residue identifications using visible-light microscopy (VLM) has led to the development of additional techniques for their characterization. Reflectance-based Fourier-transform infrared microspectroscopy (FTIRM) is a technique with great potential to aid in non-destructive residue identifications. Recent applications of the technique, however, have been hampered by methodological challenges, causing the infrared signals to be dominated by the stone rather than the residues. We address this problem by systematically testing the limits of FTIRM on five categories of experimental plant residues (wood bark, wood pith, grass leaves, starch, and resin). We demonstrate that it is possible to obtain FTIRM spectra of in situ plant residues wherein the effect of the stone is virtually eliminated. We also generate reflectance FTIRM spectral standards for each plant residue investigated and provide peak assignments for the major peaks in all spectra. The sensitivity of the technique means that slight differences in sample preparation can result in spectral differences as well. This means that archaeological application of the technique will require (1) careful, peak-by-peak analyses of the results, (2) extensive spectral libraries, and (3) research into the effects of decomposition.

Publication date: February 2017Source:Journal of Archaeological Science, Volume 78 Author(s): Mikkel Schubert, Marjan Mashkour, Charleen Gaunitz, Antoine Fages, Andaine Seguin-Orlando, Shiva Sheikhi, Ahmed H. Alfarhan, Saleh A. Alquraishi, Khaled A.S. Al-Rasheid, Richard Chuang, Luca Ermini, Cristina Gamba, Jaco Weinstock, Onar Vedat, Ludovic OrlandoHorses, asses and zebras, can produce first-generation F1-hybrids, despite their striking karyotypic and phenotypic differences. Such F1-hybrids are mostly infertile, but often present characters of considerable interest to breeders. They were extremely valued in antiquity, and commonly represented in art and on coinage. However, hybrids appear relatively rarely in archaeological faunal assemblages, mostly because identification based on morphometric data alone is extremely difficult. Here, we developed a methodological framework that exploits high-throughput sequencing data retrieved from archaeological material to identify F1-equine hybrids. Our computational methodology is distributed in the open-source Zonkey pipeline, now part of PALEOMIX (https://github.com/MikkelSchubert/paleomix), together with full documentation and examples. Using both synthetic and real sequence datasets, from living and ancient F1-hybrids, we find that Zonkey shows high sensitivity and specificity, even with limited sequencing efforts. Zonkey is thus well suited to the identification of equine F1-hybrids in the archaeological record, even in cases where DNA preservation is limited. Zonkey can also help determine the sex of ancient animals, and allows species identification, which advantageously complements morphological data in cases where material is fragmentary and/or multiple candidate equine species coexisted in sympatry.

Publication date: February 2017Source:Journal of Archaeological Science, Volume 78 Author(s): A. Blomme, P. Degryse, E. Dotsika, D. Ignatiadou, A. Longinelli, A. SilvestriGlass objects from Pydna and Methoni in modern-day Greece, dated to the eighth to fourth century BC, were chemically investigated. The combined use of multiple analytical techniques allowed the elemental and isotopic characterization of these polychrome and colourless glass artefacts in order to examine their provenance. All fragments were found to be soda-lime-silica natron-based glass produced from a rather pure silica-rich sand containing sea shells, and mixed with natron possibly coming from more than one source. Based on the strontium and neodymium isotopic signatures, most glass artefacts likely derive from a Syro-Palestinian production site although the exact location is unknown. Also the oxygen isotopic signature of most of the samples suggests the manufacturing of the artefacts from raw materials with a primary origin along the Syro-Palestinian coast. Nevertheless, the use of particular raw materials cannot be excluded for some artefacts, as some samples show enriched δ18O values pointing to a different glassmaking tradition.

Publication date: February 2017Source:Journal of Archaeological Science, Volume 78 Author(s): A.F. Greene, C.W. Hartley, P.N. Doumani Dupuy, M. ChinanderArchaeologists collaborating with material scientists at Argonne National Laboratory (ANL) as part of the Making of Ancient Eurasia (MAE) Project have developed formal methodological standards for the assemblage-based digital radiographic (DR) analysis of archaeological pottery. While analog radiography of pottery (X-radiography, Xeroradiography, etc.) has functioned as a common disciplinary tool for some time, inaccessibility, obsolescence, and significantly enhanced functionality have made DR instrumentation increasingly attractive and vital. This article presents the theoretical underpinnings, technique development, and resultant protocols that allow digital radiography to analyze very large assemblages and provide quantitative data sets that act as true counterparts to geochemical and mineralogical ones. As a technique of structural pottery evaluation, DR is particularly suited to the analysis of ceramic paste preparation and vessel formation, providing lines of evidence that can flesh out neglected portions of the chaîne opératoire, augment existing geochemical or typological classifications, and help more deeply characterize various potting traditions. Such datasets are most useful to scholars interested in harnessing the ability of the pottery “life cycle” to shed light on economic life, learning frameworks, and human social differences and group identities. The technical capacities and analytical potential of DR are demonstrated through several test analyses of ancient Chinese pottery, to be followed by more extensive case studies in draft. Prospects for closely related, three-dimensional X-ray computed tomographic approaches are also discussed.

Publication date: February 2017Source:Journal of Archaeological Science, Volume 78 Author(s): Ester Oras, Alexandre Lucquin, Lembi Lõugas, Mari Tõrv, Aivar Kriiska, Oliver E. CraigPottery was adopted by hunter-gatherers in the Eastern Baltic at the end of the 6th millennium cal BC. To examine the motivations for this cultural and technological shift, here we report the organic residue analysis of ceramic vessels from the earliest pottery horizon (Narva) in this region. A combined approach using GC-MS, GC-C-IRMS and bulk IRMS of residues absorbed into the ceramic and charred surface deposits was employed. The results show that despite variable preservation, Narva ceramic vessels were preferentially used for processing aquatic products. We argue that pottery was part of a new Late Mesolithic subsistence strategy which included more intensive exploitation of aquatic foods and may have had important implications, such as increased sedentism and population growth.

Publication date: February 2017Source:Journal of Archaeological Science, Volume 78 Author(s): Rob Wilson, David Wilson, Miloš Rydval, Anne Crone, Ulf Büntgen, Sylvie Clark, Janet Ehmer, Emma Forbes, Mauricio Fuentes, Björn E. Gunnarson, Hans W. Linderholm, Kurt Nicolussi, Cheryl Wood, Coralie MillsDendroarchaeology almost exclusively uses ring-width (RW) data for dating historical structures and artefacts. Such data can be used to date tree-ring sequences when regional climate dominates RW variability. However, the signal in RW data can be obscured due to site specific ecological influences (natural and anthropogenic) that impact crossdating success. In this paper, using data from Scotland, we introduce a novel tree-ring parameter (Blue Intensity – BI) and explore its utility for facilitating dendro-historical dating of conifer samples. BI is similar to latewood density as they both reflect the combined hemicellulose, cellulose and lignin content in the latewood cell walls of conifer species and the amount of these compounds is strongly controlled, at least for trees growing in temperature limited locations, by late summer temperatures. BI not only expresses a strong climate signal, but is also less impacted by site specific ecological influences. It can be concurrently produced with RW data from images of finely sanded conifer samples but at a significantly reduced cost compared to traditional latewood density. Our study shows that the probability of successfully crossdating historical samples is greatly increased using BI compared to RW. Furthermore, due to the large spatial extent of the summer temperature signal expressed by such data, a sparse multi-species conifer network of long BI chronologies across Europe could be used to date and loosely provenance imported material.

Publication date: February 2017Source:Journal of Archaeological Science, Volume 78 Author(s): Pauline Hanot, Claude Guintard, Sébastien Lepetz, Raphaël CornetteThe first evidence for the domestication of donkeys (Equus asinus) dates back to at least 6000-5000 BP in Northeast Africa, and their dispersion is attributed to the ancient Romans. Latin authors described donkeys as being particularly suitable for the transport of goods and farm work. In addition, they were also bred to produce prized hybrids, particularly mules, which were perfectly adapted to the long-distance transport of people and goods. However, although the historical sources extensively describe their economic importance, both donkey and hybrid remains are surprisingly scarce in the archaeological record. This apparent contradiction is probably due to the difficulties involved in correctly identifying their bones: relatively few bones displaying morphological and metrical criteria can be used for identification, so it is often based purely on bone size. The aim of this study, therefore, is to propose solutions to identify domestic equid bones using 3D geometric morphometrics on isolated and combinations of anatomical elements. A set of 3D coordinates were registered on the 18 main skull and limb bones of 111 modern reference specimens (i.e. 42 horses, 44 donkeys and 25 hybrids). In this paper, we present the classification rate obtained on this reference sample using the k-Nearest Neighbors algorithm. The application of this method on archaeological skeletons from Roman to modern sites is also presented. The percentage of correctly classified specimens was between 77% and 95% for all 18 bones, and higher than 80% for 10 of the fragmentation patterns we defined. Using a combination of several bones enabled us to increase the rate of correct reclassification to a maximum of 97%. The application to archaeological skeletons proved the ability of this method to identify domestic horses and donkeys from archaeological samples. Correspondingly, some bones, and especially combinations of bones, provided good rates to identify hybrids. This method has proved reliable in detecting the presence of donkeys and hybrids from the archaeological samples of equid bones, and should enrich our knowledge regarding their spread across Europe.

Publication date: February 2017Source:Journal of Archaeological Science, Volume 78 Author(s): Jenny Hagenblad, Jacob Morales, Matti W. Leino, Amelia C. Rodríguez-RodríguezThe Canary Islands were settled in the first millennium AD by colonizers likely originating from North Africa. The settlers developed a farming economy with barley as the main crop. Archaeological evidence suggests the islands then remained isolated until European sea-travellers discovered and colonized them during the 14th and 15th centuries. Here we report a population study of ancient DNA from twenty-one archaeobotanical barley grains from Gran Canaria dating from 1050 to 1440 cal AD. The material showed exceptional DNA preservation and genotyping was carried out for 99 single nucleotide markers. In addition 101 extant landrace accessions from the Canary Islands and the western Mediterranean were genotyped. The archaeological material showed high genetic similarity to extant landraces from the Canary Islands. In contrast, accessions from the Canary Islands were highly differentiated from both Iberian and North African mainland barley. Within the Canary Islands, landraces from the easternmost islands were genetically differentiated from landraces from the western islands, corroborating the presence of pre-Hispanic barley cultivation on Lanzarote. The results demonstrate the potential of population genetic analyses of ancient DNA. They support the hypothesis of an original colonization, possibly from present day Morocco, and subsequent isolation of the islands and reveal a farmer fidelity to the local barley that has lasted for centuries.